…infected (mean=0.53-0.39AU) and non-infected animals (mean=0.49-0.4AU). In acute SIVmac infection, intestinal IgA levels (mean=0.5-0.47AU) were similar to those of uninfected MAC (mean=0.54-0.42AU), but strongly decreased in chronically infected animals (mean=0.12-0.05AU)….
…and immunohistochemistry; plasma viral load was measured by single copy assay; specific cellular immune responses to SIV antigens were monitored flow-cytometrically by intracellular cytokine staining after stimulation with SIVsab peptides….
…or intravenous (IV) vaccination combined with a latency reversal agent (LRA) and PD-1 blockade in SIV infected macaques. Methods: A total of 28 RMs were infected with SIVmac251, placed on…
Background: Chronic SIV/HIV infection promotes an accumulation of virus-infected T follicular helper cells (T-FH cells, PD-1hi, CXCR5+, Bcl6+) in the lymph nodes, an important site of viral replication. However, the…
…(70%) patients. The other 84 patients had positive culture from skin lesions or lymph nodes. Common opportunistic infections in control patients included cryptococcal meningitis (N=83), oral or esophageal candidiasis (N=47),…
…served as treatment-naïve, ART-only controls. Plasma viral loads were measured longitudinally for 16 weeks and the reservoir size was estimated in PBMC and lymph nodes (LN) by IPDA. CD8 T…
Background: Programmed cell death protein 1 (PD-1) is an immune checkpoint marker expressed on memory T cells and enriched in latently-infected CD4+ T cells and T follicular helper cells (TFH)…
…dual bNAb and ART regimens than the VRC01 regimen. Plasma bNAb concentrations exceeded IC80 values for at least 7 days in all animals. Proviral DNA in lymph node was also…
Follicular Helper CD4+ (TFh) are antigen experienced T cells found in secondary lymphoid organs such as lymph nodes (LN). Recently, different TFh subsets has been described during chronic SIV infection…
…comprehensive scRNAseq-based approach that increases identification of HIV- transcribing cells, enabling a deep characterization of these cells. Methods: We developed HIV-seq, an approach whereby HIV primers targeting multiple regions of…