…like central memory (Tcm), which harbor latent provirus. Tissue-specific sources include lymphoid tissues (lymph nodes, spleen, and gut-associated lymphoid tissue), where virus can persist despite ART. The central nervous system…
…RNA+ cells did not differ significantly in F vs EF of LN (median F:EF 1.2, p=0.6), but were higher in F vs EF in spleen (median F:EF 3.7, p=0.03) and…
…tissues by qPCR. In situ hybridization (ISH) for SIV RNA was performed in samples from brain and mesenteric lymph node. Resting CD4+ T cells were collected before and after latency…
…were confirmed using RT-qPCR and Sanger Sequencing. CD4+ T-cells isolated from peripheral blood lymphocytes, splenocytes, thymocytes, and lymph nodes expressed human CD4 and CCR5 cell receptors in the correct orientation. …
…the ML phylogeny. All sequences originated from Glasgow and contained E138A and V179E. Mean genetic distance was <1% with multiple sets of identical sequences from different patients. Short branches in…
…and increased on total and SIV-specific effector memory CD8+ T cells during chronic infection. In addition, the level of LAIR-1 expression in the lymph node during chronic infection was higher…
…to COVID-19 in people living with HIV (PLHIV) but the precise mechanisms are still unknown. Methods Our group was amongst few that established the rhesus macaque model of COVID-19 infection…
…as lymph nodes (LN). Here, we hypothesized that LN HIV-specific CD8 T cells may harbor tissue-specific, fast-acting additional cytotoxic functions. Methods To test this hypothesis, we performed an in-depth characterization of the…
…increase over time. We found that the of reservoir of vDNA+ cells was established as early as F1 and did not decay with ART. Further, inflammatory damage occurred (as measured…
…lymph nodes. Pinpointing the anatomic sites of viral rebound can facilitate the identification of the mechanisms that specifically mediate these reactivation events in these specific tissues. Together, these observations may…