…12-plex panels) and spatial transcriptomic (GeoMx) methodologies while LN-derived cells were investigated by multiparametric flow cytometry and scRNA-10X. The blood HIV DNA content was measured by the Rainbow platform that…
…Fisher’s). Except for lymph node naïve cells, genome-intact HIV DNA was detected in all analyzed CD4 T cell subsets from both blood and lymph nodes. In LN, proportions of intact…
The mechanisms underlying abnormalities in B cell maturation and antibody response in HIV-1 infection have been only partially elucidated. The large majority of the studies have been performed in blood…
…in naive, central memory, transitional memory, and effector/effector memory CD4 T-cells. qVOA in total CD4 T cells was also negative. Peptide stimulation assays showed CCR5-negative HIV-specific CTL with loss of…
…after rebound (median VL 775 cpm; n=8), we performed intracellular cytokine staining (by flow cytometry with overlapping peptide pools) and CyTOF on PBMCs. In a second study (NCT04359186), we performed…
…reduce the follicular viral burden. We analyzed PBMCs from chronic HIV+ ART-naïve, HIV+ ART-treated and HIV- individuals, as well as lymphatic tissue from tonsils and lymph nodes of HIV+ and…
…reduce the follicular viral burden. We analyzed PBMCs from chronic HIV+ ART-naïve, HIV+ ART-treated and HIV- individuals, as well as lymphatic tissue from tonsils and lymph nodes of HIV+ and…
…and were necropsied on day 0, 3, 7 and 10 following infection. At necropsy, multiple tissues including colon, spleen, mesenteric lymph nodes and pelvic lymph nodes were obtained. 7 monkeys…
…of 80.3 in lung, 45.3 in lymph nodes, and 71.6 in spleen. Parent-to-M3 metabolite ratios in mouse tissues were 0.23 in lung, 0.40 in lymph nodes, 0.21 in spleen and…
…prevalence of TS reaching 41% (figure, node 5) TS compared to 1% (node 2) among those without anxiety irrespective of depressive symptoms. For men, the prevalence of TS among those…