…We used a novel saturation mutagenesis approach, EMPIRIC (Exceedingly Meticulous and Parallel Investigation of Randomized Individual Codons) to investigate effects of the CD4 binding loop on trimer structure and identify…
Background Enhancing immune-mediated clearance of latently infected cells is a key strategy to achieving an HIV cure. Here we evaluated CD8+ T cell responses to a potent vaccine regimen administered…
…sites is important for future eradication strategies. Near full-length proviral sequences were obtained from naïve (NV), central (CM), transitional (TM) and effector memory (EM) CD4+ T cells (sorted based on…
…in vivo dose-response study to evaluate TriKE safety and impact on NK cell responses. Methods TriKE was administered subcutaneously 5 times per week for 3 weeks in 3 uninfected RMs,…
…fusion was measured by the conventional Vpr-BlaM assay. To investigate if CD169+ myeloid cells are productively infected in vivo, lymph nodes (LNs) from pigtailed macaques chronically infected with RT-SHIVmne027 were…
…envelope glycoprotein (env) from this cohort. Methods Full length HIV-1 env was PCR amplified from extracted DNA from bone marrow aspirate (BM) and trephine (BMT), brain, bronchoalveolar lavage (BAL), cervicovaginal lavage, kidney,…
…Methods In this study, flow cytometry, cytokine array, and single-cell RNA-Seq/ATAC-Seq were conducted on SIV-infected, virologically suppressed rhesus macaques (RMs) treated with anti-IL-10 and anti-PD-1 antibodies (combo-treatment) before analytical treatment…
…Here, we performed single cell analyses to determine signaling pathways by which MA induces immune dysfunction, potentially driving viral transcription in PWH even during ART suppression. Methods Longitudinal inguinal lymph…
…next-generation sequencing approach for the direct ex vivo phenotypic profiling of individual HIV-1 infected memory CD4 (mCD4) T cells from peripheral blood (PB) and lymph nodes (LN) of people living…
…2 wpi (Intermediate, n=10), or 8 wpi (Late, n=10). Analytical treatment interruption (ATI) was performed after 1 yr. Peripheral blood (PB), lymph nodes (LN), and rectal biopsies (RB) were collected…