…and unsuppressed adult persons with HIV (PWH) to characterise viral reservoirs. Methods: Admitted adult PWH who died at Klerksdorp Tshepong Hospital, North West Province, South Africa were included in this…
…whole body and identify the location of viral reservoirs that lead to HIV-1 recrudescence are urgently needed. Methods We first performed longitudinal PET imaging using [89Zr]-VRC01 to detect HIV-1 gp120…
…and sorted by FACS from the PBMCs, bronchoalveolar lavage (BAL) lymph nodes (LN), liver, spleen, colon and jejunum using MHCI Pentamers. Next generation sequencing of TCR beta genes was performed…
…was found in lymph nodes from all hetIL-15-treated macaques. CM9 tetramer staining demonstrated that the increase of CD8+ effector T cells in lymphoid organs included actively proliferating SIV-specific T cells…
…T cells (Tfc) can locate to B cell follicles of secondary lymphoid tissue and might contribute to viremia control. Here we aimed to clarify the role of viral-specific Tfc cells…
…T cells (Tfc) can locate to B cell follicles of secondary lymphoid tissue and might contribute to viremia control. Here we aimed to clarify the role of viral-specific Tfc cells…
…of this clonal variant. Methods: Plasma and peripheral blood mononuclear cells (PBMC) were obtained ante mortem, and samples from spleen, ileum, rectum, 5 lymph nodes, and 5 tumor metastases were…
…we processed LG34’s spleen and lymph nodes post-mortem, and performed multiomic HIV-seq to characterize HIV RNA+ cells through scRNAseq and VDJ-seq to evaluate SARS-CoV-2 specificities. Results We identified 49 and…
…directed to devising strategies to eliminate or greatly reduce these reservoirs so it would be possible to discontinue ART for extended or indefinite periods of time, referred to as a…
Background: Single cell methods enhance the resolution at which infected cells in blood and tissues can be studied in people with HIV (PWH). Capturing the native state of infected cells…