…undetectable RBD antibodies served as controls. T-cell responses were quantified using activation-induced marker (AIM) assay after stimulation with SARS-CoV-2 peptide ‘megapools’ (MP): CD4 MP_S with 253 spike-spanning peptides, CD4 MP_R…
…peptide was used to prevent binding of ACBP to this receptor, as described by Dr Kroemer’s group. Results ACBP levels were higher in PLWH on ART compared to controls (median…
…these responses. Methods: Participants received gag+pol+IL-12 DNA (GP, n=11), gag+pol+env+IL-12 DNA (GPE, n=15), or placebo (n=13) vaccination via intramuscular electroporation. Individual Gag peptide-specific T cell responses (vaccine-matched 15-mer peptides) were…
…DfSO). We measured the breadth and magnitude of IFN-y T-cell responses by ELISpot assay using a 15-mer overlapping peptide (OLP) library of 2,790 SARS-CoV-2 peptides in 100 pools using a…
…in LN were higher in GC (median, 2.95 cells/mm2) than in non-GC F (median, 1.38 cells/mm2; p= 0.0008) and EF (median, 0.13 cells/mm2; p< 0.0001). A median of 55% of…
…been neglected. In this context, we and others have recently shown that gut memory CD4 T cells, lymph node (LN) T follicular helper cells and tissue macrophages represent major HIV/SIV…
…measured by RNAscope®. Cell-associated HIV RNA and total HIV DNA were measured by PCR. ARV levels were measured by liquid chromatography, tandem mass spectrometry. Participants, median age 27 yrs, were…
…(GCs) within lymph nodes (LNs) are immune privileged sites with the potential to support low level virus replication. However, the persistence of replicating virus within this tissue compartment during cART…
…pathogenesis. It has been communicated that HIV provokes alteration in phenotype and location of CD8 T-cells within lymph nodes (LN). In a mouse model, a cooperative activity between pDCs and…
…evaluated using QuPath, and multiplex phenotyping was corroborated by flow cytometry. Results Using multiplex imaging, we identified a non-T cell (CD3-) population of cells harboring SIV genomes that formed unique…