…single genome sequencing of circulating viral envs, and plasma Abs were functionally characterized. We performed flow-cytometry phenotyping of lymph node (LN)-derived germinal center (GC) cells, and single-cell transcriptomics of peripheral…
Background: CD25+FoxP3+CD4+ regulatory T (Treg) cells help mediate antigen tolerance in immune responses. We aimed to dissect the influence of anti-CD25 monoclonal antibody (mAb) on the germinal center response to…
…DNA intermediates by interferon gamma Inducible protein 16 (IFI16). Death occurs following caspase-1 activation in inflammasomes and the induction of pyroptosis, a highly inflammatory form of programmed cell death. These…
…connectivity and derive biologically meaningful brain network metrics. In this study, we use resting state functional connectivity with graph theory to evaluate participants in early HIV infection to investigate network…
…triggering intrinsic antiviral immunity in monocytes and CD4 T cells resulting in lack of viral re-seeding post-ATI (Analytical Treatment Interruption) and by boosting innate and adaptive cellular effector function. Methods:…
…question, we investigated methylation at the single-proviral level in known CpG islands in the HIV-1 proviral genome, including one in the 5′ LTR promotor region. Using a bisulfite-based, methylation-specific single-genome-sequencing…
…times for each node revealed that the median interval between transmission events within these networks were short, namely 0.9 years (0.1 – 4.7) in subtype B, 0.75 years (0.1 –…
…immune activation. Methods: Tonsil biopsies from 27 HIV-infected patients were examined. All patients had been on cART for a minimum of two years, had CD4 nadir <250 cells/µL, and HIV…
…performed using the Bayesian coalescent model as implemented in BEAST2 software. The temporal signal was estimated through the root-to-tip method using node density in tempEst v1.5.3 and the correlation coefficient…
…doses of both 10-1074 and 3BNC117 (n=8) or the vehicle control (n=8). Plasma SHIV RNA levels were measured and viral DNA were quantified in PBMC, colon and lymph node (LN) biopsies…