Background. The secondary lymphoid tissues (LT), lymph nodes (LN) and gut-associated lymphoid tissue (GALT), are the primary sites of HIV replication and where the latent pool of virus is maintained….
…we performed viral ASAPseq on purified lymph node CD4+ T cells at day 13 post-SIVmac251 infection. Finally, we developed a customized bioinformatics analysis pipeline to assess infected cells after viral…
…behavior of men who have sex with men (MSM) rather than with infection itself. Given that MSM are at higher risk of becoming infected with HIV, it is of importance…
…ART treated subjects. Definition of B and T cell populations and cytokines was performed by mass cytometry using a panel of 40 metal-conjugated antibodies. To dissect the heterogeneity of Tfh…
…Peripheral blood and lymph node (LN) samples were collected for flow cytometry analyses and viral load quantification. We observed near complete redistribution of circulating T cells into tissues within 28…
…cells in blood vs. tissues, and between latent cells reactivatable by different stimulation methods, were identified. Analysis of longitudinal samples suggested the phenotype of latent cells is stable over time.…
…vaccination against HIV remains understudied. Therefore, we aimed to investigate whether DC-based vaccination affects NK cell frequency and phenotype by analysing samples from a previously performed therapeutic vaccination study (Allard…
…on cell-associated (CA)-RNA, we used Analysis of Compositions of Microbiomes with Bias Correction (bacterial taxa) and linear regression models (metabolites). Results: Irrespective of the regimen and duration of treatment, the…
…3-18 years of ART. We also examined the genetic similarity of HIV sequences found in T cell subsets and plasma viremia. Using single-genome/proviral sequencing, we performed cross-sectional inter-participant analysis of…
…compared to pMHC- RM (mean log10 6.7 vs 7.2; p=0.003) and pMHC+ RM achieved PVL suppression (15 copies/ml) more rapidly than pMHC- RM (median 6 wpi vs 15 wpi, p=0.002)….