Using dual-photon in-vivo imaging, we previously showed significantly lower concentrations of tenofovir (TFV) in rat lymph nodes (LN) compared to peripheral blood (PB). This finding was later confirmed for intracellular…
…DRV, 4 ATV), with a subset with ileal (2 RAL, 1 DRV, 1 ATV) and lymph node (2 RAL, 1 DRV) samples. The median age was 44 years and median…
…Medicine. A portion of each LN was formalin-fixed, paraffin-embedded (FFPE) and slide-mounted tissue sections were prepared. H&E staining was performed on LN slides and fibrotic regions were quantified by a…
…detectable, although LC-MS/MS confirmed TFV-dp and FTC-tp concentrations exceeding EC50 (36.7 and 29.6 fmol/106 cells, respectively); so spatial analysis focused on 2DR. Spatial measurement of DTG+3TC indicated exposure of ≥1…
…vDNA and vRNA in CD4 T cells and lymph nodes (LN). Methods We randomized 12 participants in AHI to receive immediate ART (n=4, Fiebig 1-2) or ART + N-803 (n=8,…
…disseminated across lymph node, spleen, and kidney. Ten large clones of defective proviruses were also noticed, which were broadly distributed across lymph node, spleen, the CNS, the genitourinary system, and…
…We examined viral protein-specific CD8+ T-cell responses in these PBMCs and LN-derived lymphocytes by detection of IFN-γ induction after stimulation with overlapping peptide pools and performed correlation analyses between these…
…or maraviroc (MVC) + atazanavir (ATZ) (N=3), cohort 2. Tissue accumulation of ARVs and metabolites was measured by LC-MS/MS (analyte-specific limits of detection [LOD]: 1-8 ng/ml homogenate) and infrared matrix-assisted…
…(median days after diagnosis =424, IQR 141-719; median days on ART =899, IQR 405-1567), 5 during acute infection (AT) (median days after diagnosis =1, IQR 0.5-1; median days on ART…
…both active and repressive chromatin compared with PBMC-derived CD4⁺ T cells (H3K27ac: p=0.0015; H3K9me3: p<0.0001; H3K27me3: p=0.0007). Conclusions This study provides the first evidence that LN-derived Tfh cells harbor clonally…