…cytometry. HIV-specific T cell immunity was determined by IFNγ ELISPOT in response to Gag, Pol, Env, Nef/Tat/Rev, Vpr/Vpf/Vpu peptide pools as well as CMV-pp65 and EBV BZLF-1 peptide pools. Frequencies…
…performed epitope mapping to dissect anti-HIV-1 responses and correlations between vaccine-induced responses and maximum VL post-ATI. PBMC from 16 participants were incubated with 36 (15-mer) and 56 (9-mer) overlapping peptides…
…the peptide microarray, a multiplex platform to measure antibody binding to 6,654 peptides from across the HIV proteome and covering the majority of global sequences. Positive peptide responses were tallied…
…vivo using specific antigens, we aimed to show that latency is preferentially established in cells with certain antigen-specificities. We used peptide pools of HIV-1 Gag, CMV, C. albicans, and a…
…after receiving the PHH-1V booster. PBMCs were isolated and stimulated with 15-mer overlapping peptides pools (84 peptides) covering the RBD region of the Spike protein from the RBD Omicron BA.1…
…CD103. Furthermore, ‘dirty’ mice exhibited substantially more of the TRM phenotype within lymph nodes than SPF mice. Lymphoid TRMs are detectable in HIV-infected lymph nodes using histo-cytometry and most CD8+…
…Inguinal lymph node (LN) excisions were performed by a board-certified surgeon on 6 virally-suppressed HIV-infected participants on ART and 4 HIV-uninfected participants recruited at the University Clinics at Kaka’ako at…
…for plasma time-course and intracellular drug levels in lymph nodes. Methodology: The LNPs composed of lipids (DSPC: mPEG2000-DSPE) (8:2 m/m); and anti-HIV drugs-LPV, RTV and RNFV (115:10:5:15 lipids:LPV:RTV:TNFV m/m/m/m) were…
…n=130/484) were grouped in 54 clusters: 39 clusters with 2 nodes, 11 clusters with 3 nodes, 2 clusters with 4 nodes, 1 cluster with 5 nodes and 1 cluster with…
…Methods: We used a highly processive and accurate polymerase and PacBio sequencing to obtain near full-length (NFL) genome sequences from blood, lymph node, and gut biopsies in 5 SHIV-infected rhesus…