…was associated with a transmission clustering pattern. We present the possibility of identifying LHD-involving clusters in transmission-network analysis using our large-scale monitoring of domestic transmission clusters (dTCs) in Japan. Methods:…
Background: Gastrointestinal (GI) integrity/permeability were investigated as mechanisms for T-cell activation and poor immune recovery during cART (Immunological Non Responders, INR). Methodology: HIV+ patients (pts) on suppressive cART for at…
…Tissue was digested into single-cell suspensions for measurements of T-cell subsets and activation phenotype by immunophenotypin Results: Twenty-six HIV+ subjects completed the follow-up. Median age was 37 years [25-42] vs…
…associations with virological and immunological parameters. Methodology: Colonic mucosal cells (CMC) and peripheral blood (PB) mononuclear cells were obtained from 24 untreated HIV-1 infected (HIV-1+; median viral load (VL): 51,350…
…permissiveness, we performed a genome-wide analysis of gene expression in RA-treated CCR6+ versus CCR6- T-cells. Methodology: CD4+ T-cells were sorted from PBMCs by negative selection using magnetic beads (Miltenyi). Memory…
…pharmaceutical agents capable of safely reactivating latent HIV-1 in ART-suppressed patients. Methods: A study was conducted in SIV-infected rhesus macaques (RM) on ART to determine if administration of an oral…
…to any other blood and lymph node (LN) memory CD4 T-cell populations. On the basis of this observation, we hypothesized that increased HIV transcription in Tfh cells may be facilitated…
…in iron metabolism and ferroptosis resistance was evaluated by RNA-seq, qPCR, immunoblot, FACS and IFA. Cellular iron content was measured by ICP-MS. Markers of pro/antioxidant status (e.g., ROS, GSH) were…
Identifying cellular subsets that preferentially harbour HIV is important for curative strategies. We aimed to address the role of the immune checkpoints, programmed cell death 1 (PD1) and cytotoxic T-lymphocyte…
…fusion was measured by the conventional Vpr-BlaM assay. To investigate if CD169+ myeloid cells are productively infected in vivo, lymph nodes (LNs) from pigtailed macaques chronically infected with RT-SHIVmne027 were…