…lymph nodes, their role in restricting early HIV-1 replication and the mechanisms by which they mediate their antiviral activity are poorly understood. We recently demonstrated that transmitted-founder (TF) viruses are…
…mechanisms underlying microbial translocation. To characterize translocating bacterial populations, we isolated and identified translocating bacteria from chronically SIV-infected macaques and characterized their genomes and proteomes. Proteome profiling identified a cytosine-specific…
…lymph nodes. Pinpointing the anatomic sites of viral rebound can facilitate the identification of the mechanisms that specifically mediate these reactivation events in these specific tissues. Together, these observations may…
…those in blood and peripheral lymph nodes. CNS histopathology were analyzed by immunohistochemistry in combination with in situ hybridization. Transcriptomic analyses were performed on brain tissue. Results: While anti-mRh-IL-15 treatment…
…RNA quantified by RT PCR. Data were log transformed. Mixed-effects models and repeated-measures ANOVA were used with pairwise tests if overall p<0.05. Geometric means and 95% CIs are reported. GFP…
…populations was evaluated by high-throughput single genome amplification using the PacBio platform to sequence Full-length HIV envelope (FL HIV-env). We evaluated post-mortem tissues from 9 men and 3 women with…
Background Fingolimod, immunomodulating drug indicated for people with multiple sclerosis (MS), is a sphingosine-1-phosphate-receptor modulator that reduces CD4+Tcells levels by lymph node sequestration. In vitro, fingolimod decreases HIV infection and…
…Cytokines and soluble cell death mediators were analyzed by Luminex. Results: Phenotypic analysis of tonsillar cells revealed a memory population of CD8 T cells expressing a CCR7lowCXCR5high profile compatible with…
…resting memory CD4+ T-cells support a stable latent reservoir (LR) of integrated virus. However, Lorenzo-Redondo et al. [Nature, 2016] deep-sequenced lymph node and blood samples from three participants during the…
…effects of Interleukin (IL)-21 administration in chronic, ART-treated SIV-infected rhesus macaques (RMs) on mucosal integrity, residual inflammation, and virus persistence. Methods: Sixteen RMs were infected with SIVmac239 i.v. and, starting…