…node mononuclear cells (LNMC) from the vaccinated animals stimulated ex vivo with SEB and IFNγ was measured by ELISA, multiplex chemiluminescence detection assays (MSD) and intracellular staining. High magnitude (up…
…trend was seen for TFR within follicles (medians, 59 versus 22 cells/mm2; p=0.10). In ART treated HIV+, Tregs (median, 36 cells/mm2) and TFR (median, 76 cells/mm2) did not differ significantly…
Identification of live HIV-infected CD4 cells allows the characterization of individual cells both by flow cytometry and by transcriptomic analysis. Cervical lymph nodes were obtained from 3 viremic HIV-infected volunteers, median…
…samples that can be obtained from patients, we developed a model using SIV-infected rhesus macaques. The methods of Maldarelli et al (Science 345: 179, 2014) were used to generate an…
…of Granzyme B and exert a higher capacity to mediate in vitro bispecific-mediated redirected killing of infected cells. Chronically infected AGM, on the other hand, did not accumulate fCD8. Fluidigm…
…care. Our aim was to understand uptake of this intervention and related patient-level factors, which is critical to improving the PrEP cascade. We analyzed medical record data for HIV-negative men…
…anti-gp41 Adnectin was joined at its amino terminus to the anti-CD4 Adnectin via a peptide linker. A third inhibitor, an alpha-helical peptide fusion inhibitor, was linked to the carboxy end…
…co-expressed gag and nef RNA. Reactivated cells were dominated by transcriptionally heterogeneous vRNA+p24- cells: gag+nef- (median 47%) > gag-nef+ (median 30%) > gag+nef+ (median 12%). PEP005 and panobinostat resulted in…
…placebo recipients were tested in ELISA at a 1:100 dilution for reactivity vs. biotinylated cyclic V3 peptides (cV3s). Results: Of 8 cyclic V3 peptides studied, reactivity was strongest vs. clade…
Current guidelines recommend 4 weeks of daily ARVs for post-exposure prophylaxis (PEP) after an HIV exposure, though the optimal duration of PEP is not known. An effective short-course regimen could…