…or HIV+ donor leukapheresis samples. Autologous HSTs were generated by stimulating T-cells with pools of overlapping Clade B consensus peptides or peptides representing only conserved viral epitopes. Four to six…
…IPV was 45% – >82000 women. Lifetime prevalence of NP-rape was 18% – >28000 women and girls. Very few sought care – 5% told a health care professional about their…
…on CMV specific effector cells. Methodology: Peripheral blood mononuclear cells (PBMCs) obtained from HIV seronegative, CMV seropositive healthy donors were cultured in presence of heat inactive HIVBaL. After 5 days,…
…pp65 peptide pool, Mtb ESAT-6/CFP-10 peptide pool, and whole cell Mtb lysate (WCTBL). Cells incubated in media alone served as a negative control. PBMCs were stained for markers of T…
…VIHGILA project, implemented in 25 hospitals in Catalonia (Spain), has aimed to proactively diagnose HIV infection across six clinical scenarios: suspected sexually transmitted infection (STI), chemsex, post-exposure prophylaxis (PEP) request,…
…enzyme that mediates the cleavage of Gag, Gag-Pol and Nef precursor polyproteins. This process is a highly specific, temporally regulated and essential for the production of infectious viral particles. A…
…appropriate. CD4+ T cells expressing CD32 were highly resistant to ADCC after peptide-loading (ANOVA p=0.0001) (Figure 1), and ex-vivo infection (median % 19.6 vs. 35.8 for TCD32+ and TCD32-, p…
…with Spike (S) and Nucleocapside (N) SARS-COV-2 peptide pools. Likewise, an optimized peptide pool of HCoV S protein was used in HD. T-cell polyfunctionality by intracellular cytokine staining (IFN-?, IL-2,…
…cell-mediated immune responses (CMI); and HLA typing. CMI were assessed using pooled clade-matched HIV-1 Gag peptide panels in matrix format and IFN-g ELISpot and expressed as spot-forming units (SFU) per…
…production after whole blood stimulation with SARS-CoV-2-spike peptides, HHV peptides designed to elicit CD4/CD8 response for Varicella Zoster Virus (VZV), Cytomegalovirus (CMV), HHV1/2, Epstein-Barr virus (EBV), and IL-2, IL-6, IL-8,…