…vitro proteolysis reactions were performed +/- RNA with globular substrates derived from Gag or a 12-amino acid peptide. HIV PRs of multiple lineages were used to investigate whether RNA-enhancement is…
…cells, identified by IFNγ production following CMV peptide pool stimulation (pp65 and IE-1 pools, JPT Peptide Technologies). We used Mann-Whitney tests and nonparametric Spearman correlations to evaluate p and r…
Since 2008, Kenya has implemented medical male circumcision for HIV prevention, with passive reporting of moderate and severe adverse events (AE) to monitor program safety. With PEPFAR support, an additional…
…peripheral blood samples of 27 untreated individuals with HIV. Isolated peripheral blood mononuclear cells were labeled with fluorescently tagged HLA-peptide tetramers presenting immunodominant epitopes of the HIV proteome (Figure 1)…
…pp65 peptide pool, Mtb ESAT-6/CFP-10 peptide pool, and whole cell Mtb lysate (WCTBL). Cells incubated in media alone served as a negative control. PBMCs were stained for markers of T…
…following a 6-hour peptide stimulation, and scRNAseq was performed for TCR repertoire analysis. T cell lines were generated by expressing recombinant TCRs in Jurkat cells and activation was measured by…
…a surface activation induced marker (AIM) assay using a CD4-optimized overlapping SARS-CoV-2 peptide pool to measure OX40/CD137 expression following peptide stimulation and sorted CD4+ T cells based on surface marker…
…on rates of HIV transmission, we measure annual trends in HIV incidence, PrEP consults, PEP episodes, number of individuals screened annually, mean number of HIV tests per individual annually, and…
…(PEPSE), bacterial STIs or use of drugs in a sexual context before or during last sexual intercourse was determined using the Kaplan-Meier method. Separate analyses were performed for MSM and…
…Together with higher surface GPVI expression, these observations suggest improvements in measures of platelet function involving endothelial-platelet pathways with a switch from ABC/3TC and point to a potential underlying mechanism…