HYNES CONVENTION CENTER

Boston, Massachusetts
March 8–11, 2020

 

Conference Dates and Location: 
February 23-26, 2015 | Seattle, Washington
Abstract Number: 
49

Identification and Characterization of Individual HIV-Infected CD4 T Cells Ex Vivo

Author(s): 

Joseph Casazza1, Irene Primmer1, David Ambrozak1, Constantinos Petrovas1, Sara Ferrando-Martinez1, Perla Del Río-Estrada2, Gustavo Reyes-Terán2, Ezequiel Ruiz-Mateos3, John Mascola1, Richard A. Koup1
1 Vaccine Research Center, NIAID, NIH, Bethesda, MD, United States. 2 Instituto Nacional de Enfermedades Respiratorias, Mexico City, Mexico. 3 HU Virgen del Rocio/IBIS, Sevilla, Spain.

Abstract Body: 

Background: Identification of live HIV-infected CD4 cells would allow the characterization of these cells by flow cytometry and single cell transcriptomic analysis.

Methods: bnAbs were screened for their ability to identify individual BaL-infected primary CD4 T cells as identified by P24 intracellular staining and transcription of Gag, and D1A3 (Tat-associated) and D1A4b (Rev-associated) spliced HIV RNA. CD4 mimic, V1V2 and the V3 glycan bnAbs were found that stained BaL-infected cells. bnAbs used for ex vivo studies were selected based on breadth of neutralization of B clade virus, and sensitivity and intensity of staining of HIV-infected CD4 T cells. Live CD3+CD45RO+CD8-CD14-CD19-TCRδγ- CD4 dim and null cells prepared from lymph nodes were stained for expression of PD-1, CD57, CXCR4, CXCR5, CD27 and PGT121 and then bulk sorted into PD-1+; PD-1-CD57-; and PD-1-CD57+ populations. Individual PD1+ cells were index sorted into individual wells, RNA was extracted, purified, and Gag, and Rev- and Tat-associated RNA copy numbers determined by RT PCR. Measureable Tat- and Rev-associated HIV RNA were assumed to represent active transcription of HIV proviral DNA.

Results: The highest frequency of CD4 T cells transcribing proviral DNA was found in the PD-1+, CD4 dim and null population. PD-1+ cells were index sorted and Gag, Tat- and Rev-associated HIV RNAs measured. Ninety-one of 599 (15%) cells sorted from lymph nodes from six untreated, HIV-infected individuals, were actively transcribing HIV RNA. Eighty-five percent of the cells transcribing Tat- and Rev-associated RNA also contained measurable Gag RNA. Staining of HIV envelope by PGT121 was significantly associated with proviral DNA transcription (P<0.0001). Median percentage of PGT121+ CD4 dim and null T cells actively transcribing virus was 41 (range, 12-64)% compared to 17(3-22)% in PGT121- cells. PGT121 staining was strongly associated with downregulation of CD4 (P<0.0001). A higher percentage of CXCR5+PD1+ cells actively transcribed proviral DNA than did CXCR5-PD-1+ cells (P<0.0001). A lesser proportion of CD57+ CD4 T cells, a marker of germinal center cells, actively transcribed proviral DNA than did CD57- CD4 T cells (P<0.05).

Conclusions: Most CD4 T cells transcribing proviral DNA are Tfh cells. Viral transcription occurs both inside and outside of the germinal center in B cell follicles and is identifiable by bnAbs. bnAbs may be a means of targeting HIV-infected CD4 T cells in lymph nodes.

Session Number: 
O-4
Session Title: 
New Discoveries in HIV Pathogenesis
Presenting Author: 
Casazza, Joseph
Presenter Institution: 
Vaccine Research Center, NIAID, NIH