CONFERENCE ON RETROVIRUSES
AND OPPORTUNISTIC INFECTIONS

Boston, Massachusetts
March 8–11, 2020

 

Conference Dates and Location: 
February 22–25, 2016 | Boston, Massachusetts
Abstract Number: 
434

Contamination of Herbal Medicines With ARVs and Widespread Use by PLWH in Nigeria

Author(s): 

Joshua Gini1; Bala I. Harri2; Elkanah D. Kabilis3; Mark Stevens4; Paul P. Pama5; Alieu Amara1; Dilly P. Sujan1; Justin Chiong1; Saye Khoo1; for the Pharmacology Research Laboratories University of Liverpool
1Univ of Liverpool, Liverpool, UK;2Dalhatu Araf Specialist Hosp, Lafia, Nigeria;3Gombe State Specialist Hosp, Gombe, Nigeria;4Faith Alive Fndn, Jos Plateau State, Nigeria;5Fed Med Cntr Katsina, Katsina, Nigeria

Abstract Body: 

Nigeria has an estimated 3.1 million people living with HIV (PLWH), accounting for nearly 10% of global HIV burden. Use of traditional medicines is high in the general population. Here, we sought to evaluate the use of herbal medicines amongst (PLWH), and undertake a country-wide analysis of herbal medicines used by PLWH for possible contamination with antiretrovirals (ARV).

Part A) large questionnaire-based survey of patients attending health centres in mixed rural and urban centres across 4 states. Data were collated and analysed using SPSS.  Part B) country-wide (Fig. 1) collection of herbal samples for drug analysis. Investigators followed a standard protocol i) street vendors from a mixture of urban and rural settings were approached ii) a request for herbals for treatment of general pains, hepatitis, UTI, and febrile ailments on a background of HIV iii) herbals sold as powders or liquids were purchased iv) instructions for use, date and site were recorded. Analysis for efavirenz, nevirapine, lopinavir, darunavir, ritonavir, atazanavir, emtricitabine, tenofovir and lamivudine using validated LC-MS/MS methods was performed at the University of Liverpool.

Of 742 PLWH aged 2-91y, prevalence of herbal medication use was 41.8% (310). Use of herbals was significantly associated with educational attainment (31.8% in patients with little or no education, vs 43.8% and 44.8% in patients with secondary and tertiary education respectively; P= 0.037) and with employment status (44.9% of the employed vs 36.7% of unemployed patients used herbals; P= 0.01). Of those who took herbals, 45% did so prior to commencement of ARVs, 53.9% did so to cure HIV (46% with little effect while 5.1% believed themselves cured); influence of family or friends contributed to herbal intake in 75%.  Of 138 herbal samples collected across 8 States, 3 (2%; all from large cities) contained measurable antiretrovirals. One sample contained tenofovir (0.2ng/mg powder) and emtricitabine (0.0065ng/mg powder), while two samples contained tenofovir (0.2 and 1.6ng/mg powder) and emtricitabine (0.123 and 0.00049ng/mg powder), with one of these also containing lamivudine (0.25ng/mg powder)

Herbal use amongst PLWH is widespread, poorly recorded and often precedes ARV therapy. Contamination with ARVs is worrying (particularly in untreated patients) given the potential for drug resistance.

Session Number: 
P-H1
Session Title: 
Exposure Response: Learning to Improve Safety and Efficacy
Presenting Author: 
Joshua Gini
Presenter Institution: 
University of Liverpool
Poster: